OncoStrands® Extended > DNA Laboratories Staging Site

Specimen Requirements
Clinical usage	109-genes hybrid capture DNA NGS panel with coverage of full coding regions of 103 genes, including BRCA1 & BRCA2 and many other DNA damage repair genes (plus TERT promoter region). The assay also provides an accurate microsatellite instability (MSI) score (close to 170 loci). Useful for tumours in the prostate, breast, ovary & fallopian tube, pancreas, biliary tract, uterus and colon. NOTE: Can be ordered in combination with 18 genes Essential Fusion if screening for fusions is clinically important.
Type of sample	Formalin-fixed paraffin-embedded (FFPE) Tissue/Cell Block (preferred) or 15 x 5µm unstained slides of FFPE tissue mounted on uncoated slides + 1 H&E stained slide
Surface Area & Tumour Purity	Minimum surface area of 5 x 5 mm2 per slide. If the tissue area is smaller, please provide additional 5 – 10 5µm unstained slides. At least 20% tumour purity per slide.
Storage & transportation	Please keep at room temperature. Do not freeze, and keep away from direct sunlight.
Turnaround Time (TAT)	2 – 3 weeks

Specimen Requirements

Clinical usage

109-genes hybrid capture DNA NGS panel with coverage of full coding regions of 103 genes, including BRCA1 & BRCA2 and many other DNA damage repair genes (plus TERT promoter region). The assay also provides an accurate microsatellite instability (MSI) score (close to 170 loci). Useful for tumours in the prostate, breast, ovary & fallopian tube, pancreas, biliary tract, uterus and colon.

NOTE: Can be ordered in combination with 18 genes Essential Fusion if screening for fusions is clinically important.

Type of sample

Formalin-fixed paraffin-embedded (FFPE) Tissue/Cell Block (preferred) or 15 x 5µm unstained slides of FFPE tissue mounted on uncoated slides + 1 H&E stained slide

Surface Area & Tumour Purity

Minimum surface area of 5 x 5 mm2 per slide. If the tissue area is smaller, please provide additional 5 – 10 5µm unstained slides. At least 20% tumour purity per slide.

Storage & transportation

Please keep at room temperature. Do not freeze, and keep away from direct sunlight.

Turnaround Time (TAT)

2 – 3 weeks